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目的:探讨骨髓间充质干细胞(BMSC)移植对脑缺血大鼠神经功能恢复和脑组织中突触素(synaptophysin)mRNA表达的影响。方法:72只清洁级成年雄性SD大鼠,随机分为假手术组(Sham组)、局灶性脑缺血组(MCAO组)、溶剂对照组(vehicle组)和BMSC治疗组(BMSC组),每组18只,采用改良Zea-Longa线栓法制作大脑中动脉栓塞(MCAO)模型,BMSC移植前用DAPI标记,BMSC组于造模成功1 d后经侧脑室注射入BMSC(1×106),vehicle组则注射同等剂量的PBS,术后4 d、7 d和14 d通过平衡木行走、转棒上行走及网屏抓握进行神经功能评估,观察其恢复状况,并断头取脑,免疫组织化学及RT-PCR法检测突触素表达情况。结果:BMSC组缺血周边区脑组织中观察到DAPI染色的阳性细胞。BMSC组术后7 d和14 d神经功能评估明显优于MCAO组和vehicle组(P<0.05 orP<0.01);BMSC组术后4 d、7 d和14 d脑组织中突触素表达较MCAO组和vehicle组明显增高(P<0.01)。结论:BMSC移植可以促进脑缺血大鼠神经功能的恢复,促进缺血脑组织中突触素生成,BMSC移植促进脑缺血大鼠神经功能恢复部分是通过促进脑组织中生成突触素发挥作用。
Objective: To investigate the effects of bone marrow mesenchymal stem cells (BMSC) transplantation on the recovery of neurological function and the expression of synaptophysin mRNA in rats with cerebral ischemia. Methods: Seventy-two adult male Sprague Dawley rats were randomly divided into Sham group, MCAO group, vehicle control group and BMSC group (BMSC group) (N = 18). The model of middle cerebral artery occlusion (MCAO) was established by modified Zea-Longa method. The BMSCs were labeled with DAPI before transplantation. The rats in BMSC group were injected into BMSC (1 × 106 ), the same dose of PBS was injected into the vehicle group, and the neurological function was assessed on the 4, 7 and 14 days after operation by walking on balance beam, walking on the stick and grasping by the screen. The recovery status was observed and the brain was decapitated. The expression of synaptophysin was detected by immunohistochemistry and RT-PCR. Results: DAPI-positive cells were observed in the ischemic border area of BMSC group. The neurological function of BMSC group was significantly better than that of MCAO group and vehicle group at 7 and 14 days postoperatively (P <0.05 or P <0.01). The synaptophysin expression of BMSC group was significantly higher than that of MCAO group at 4 d, 7 d and 14 d Group and vehicle group was significantly higher (P <0.01). Conclusion: Transplantation of BMSC can promote the recovery of neurological function, promote the production of synaptophysin in ischemic brain tissue and promote the recovery of neural function in rats with BMSC transplantation partly by promoting the production of synaptophysin in brain tissue effect.