用水合联肼作还原剂研制成亲水纳米铜颗粒 ,用琥珀酸二异辛酯磺酸钠 /丙三醇 /正庚烷反胶束体系合成出憎水纳米铜颗粒 ,并通过透射电镜和紫外光谱考察了制得的纳米颗粒样品 .用憎水纳米铜颗粒及亲水纳米铜颗粒与聚乙烯醇缩丁醛构成复合固酶膜基质 ,用溶胶 凝胶法固定葡萄糖氧化酶 ,构建葡萄糖生物传感器 .实验结果表明 ,纳米铜颗粒可大幅度提高固定化酶的催化活性 ,响应电流从相应浓度的几十纳安增强到几千纳安 .从理论和实验上证明了纳米颗粒对固定酶的作用 ,讨论了纳米颗粒对酶催化性能的改善作用 ,为纳米颗粒在生物传感器领域中的应用提供了可供参考的实验和理论依据 Hydrazine hydrate was used as reductant to prepare hydrophilic nano-copper particles. Hydrophobic nano-copper particles were synthesized by sodium diisooctyl sulfosuccinate / glycerol / n-heptane reverse micelles system and analyzed by transmission electron microscopy Ultraviolet spectroscopy was used to investigate the prepared nanoparticle samples.With hydrophobic nano-copper particles and hydrophilic nano-copper particles and polyvinyl butyral to form composite enzyme-immobilized membrane matrix, immobilized glucose oxidase by sol-gel method to construct glucose biosynthesis Sensor.Experimental results show that the nano-copper particles can greatly improve the catalytic activity of immobilized enzyme, the response current from the corresponding concentration of tens of satisfied to enhance to several thousand nanoamper.From the theoretical and experimental evidence that the nanoparticles on the immobilized enzyme The role of nanoparticles to improve the catalytic performance of the enzyme discussed for the application of nanoparticles in the field of biosensors provide experimental and theoretical basis for reference