目的　建立HLA Ⅰ积累表型频率(CPF)的空间预测系统,旨在指导中国疫苗学家设计受HLA限制的多表位疫苗(包括DNA疫苗) ,并评价或预测根据不同HLA Ⅰ组合形式设计的多表位疫苗(或DNA疫苗)在中国不同地区的免疫效果。方法　通过构建中国境内人群HLA Ⅰ基因空间数据库,利用“克立格”技术建立CPF空间预测系统。结果　根据疫苗设计目的,设计者可利用该系统预测和估计特定地理位置上人群的CPF ,包括根据单个HLA座位(HLA A或HLA B)上的等位基因而设计的多表位疫苗和根据两个HLA基因座位(HLA A和HLA B)上的等位基因而设计的多表位疫苗在特定地理位置上人群的CPF值,从而估计或预测拟研制的或已研制的多表位疫苗在中国不同地理位置上人群中的免疫效果。结论　该预测系统可用于:①鉴别任何中国群体的期望CPF百分比,为设计疫苗组分提供依据;②在特定地理位置的人群中,预测限制性表位已知的多表位疫苗的理论免疫应答率;③在特定地理位置的人群中,识别因缺乏HLA Ⅰ限制性等位基因而不能对表位疫苗产生免疫应答的个体。 OBJECTIVE: To establish a spatial prediction system for HLA Ⅰ accumulation phenotype frequency (CPF) to guide Chinese vaccinators in the design of HLA-restricted multi-epitope vaccines (including DNA vaccines) and to evaluate or predict the number of HLA- Multi-epitope vaccine (or DNA vaccine) in different parts of China’s immune effect. Methods The spatial database of HLA Ⅰ gene in Chinese population was constructed, and the spatial prediction system of CPF was established by using “kriging technique”. Results Based on vaccine design goals, the designer can use this system to predict and estimate CPF for a population in a particular geographic location, including multi-epitope vaccines designed based on alleles on a single HLA seat (HLA A or HLA B) Epitopes in HLA loci (HLA A and HLA B) were designed to estimate the CPF value of the population in a specific geographical location so as to estimate or predict the potential of the multi-epitope vaccine to be developed or developed in China Immune effects in populations at different geographic locations. Conclusions The predictive system can be used to: (1) identify the expected CPF percentage of any Chinese population and provide a rationale for the design of the vaccine component; (2) predict the theoretical immune response to multi-epitope vaccines with restricted epitopes in a population of geographically specific locations Rate; ③ In populations with specific geographic locations, identify individuals who can not develop an immune response to epitope vaccines due to the lack of HLA I-restricted alleles.