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目的探讨己酮可可碱(PTX)对非乙醇性脂肪性肝炎(NASH)的治疗作用。方法将实验性脂肪性肝炎模型大鼠,随机分为正常对照组、模型组、治疗组3组。模型组、治疗组大鼠继续高脂饲养,治疗组予以PTX16mg/(kg·d)治疗4周。对照组大鼠普通饲料喂养。实验16周时处死3组大鼠,分别检测体质量、肝指数、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、三酰甘油(TG)、胆固醇(TC),并进行HE、油红O和Masson染色观察肝脏病理改变,进行炎症活动度计分。结果模型组大鼠体质量、肝指数明显高于正常对照组(P<0.01),PTX治疗后体质量及肝指数均显著降低(P<0.01);模型组大鼠血清ALT、AST与正常对照组比较差异有统计学意义(P<0.01),治疗组大鼠血清AST与模型组比较差异有统计学意义(P<0·01)。模型组TC明显高于对照组(P<0·05),治疗组TG、TC均降低,但差异无统计学意义。组织学观察显示治疗组肝组织炎症坏死程度减轻,炎症活动度计分明显下降(P<0·01)。结论PTX可改善高脂饮食诱发的脂肪性肝炎大鼠肝脏酶学及组织损伤,对实验性NASH具有治疗作用。
Objective To investigate the therapeutic effect of pentoxifylline (PTX) on non-alcoholic steatohepatitis (NASH). Methods Experimental steatohepatitis model rats were randomly divided into normal control group, model group and treatment group. The rats in the model group and the treatment group continued to be fed with high fat, and the patients in the treatment group were treated with PTX16mg / (kg · d) for 4 weeks. Control rats were fed the normal diet. After 16 weeks, the rats in each group were sacrificed and the body weight, liver index, ALT, AST, triglyceride (TG), cholesterol (TC) , Oil red O and Masson staining to observe the pathological changes of the liver, scoring inflammatory activity. Results The body weight and liver index of the model group were significantly higher than those of the normal control group (P <0.01). The body weight and liver index of the model group were significantly decreased (P <0.01). The levels of ALT and AST in the model group were significantly lower than those in the normal control group There was significant difference between the two groups (P <0.01). There was significant difference between the treatment group and the model group (P <0.01). The TC in the model group was significantly higher than that in the control group (P <0.05), while the TG and TC in the treatment group decreased, but the difference was not statistically significant. Histological observation showed that the treatment group, liver inflammation and necrosis reduce the degree of inflammatory activity was significantly decreased (P <0.01). Conclusion PTX can improve liver enzymology and tissue injury induced by high-fat diet in rats with steatohepatitis, and has a therapeutic effect on experimental NASH.