目的：培养大鼠血管平滑肌细胞。方法：手术显微镜下分离主动脉和肺动脉，再用翻转干涸法进行操作。结果：显微镜下分离组织，可基本除净动脉纤维脂肪层和外膜，再用刀片轻刮内膜，可保证所贴组织块为动脉中膜。传至第三代时，用台盼蓝检查细胞传代成活率95％，光镜、电镜和免疫组化鉴定培养细胞，培养细胞纯度96％。结论：贴块片简便易行、经济，又可以防止膜损伤，可为血管病理变化研究提供适宜的细胞。 Objective: To culture rat vascular smooth muscle cells. METHODS: Aortic and pulmonary arteries were isolated under a surgical microscope and then manipulated by tumbling and drying. Results: Microscopically, the tissue was separated, except the net fat layer and the adventitia of the arterial fiber. To the third generation, 95% of cells were passaged with trypan blue, and cultured cells were identified by light microscope, electron microscope and immunohistochemistry. The purity of cultured cells was 96%. Conclusion: Blocking tablets is simple, economical, and can prevent membrane damage, and can provide appropriate cells for the study of vascular pathological changes.