将葡萄卷叶伴随病毒 的 CP基因克隆到表达载体 p ET- 30 a中 ,转化大肠杆菌 BL2 1,筛选得到阳性克隆 GB- 1,用 IPTG诱导使其表达。SDS- PAGE电泳分析表明 ,该 CP基因在大肠杆菌 BL2 1中得到大量表达。用该病毒的 CP抗血清通过间接 EL ISA证明所表达的蛋白具有很好的抗原性 The CP gene of grapevine associated virus was cloned into the expression vector p ET-30 a and transformed into E. coli BL21. The positive clone GB-1 was screened and induced by IPTG. SDS-PAGE electrophoresis analysis showed that the CP gene was highly expressed in E. coli BL21. The CP antiserum of the virus was used to demonstrate that the expressed protein has good antigenicity by indirect ELISA