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目的建立HPLC-ELSD法测定当归补血汤配方颗粒中黄芪甲苷含量的分析方法。方法采用Agilent Zorbax C18柱(4.6mm×250mm,5-Micron),以乙腈-水为流动相,流速1.0m L/min,柱温25℃,ELSD检测器漂移管设置温度:70℃,ELSD检测器设置气体流量:2.0 L/min。结果该条件下黄芪甲苷的线性关系,精密度、重复性、稳定性均良好。平均回收率为97.17%。结论 HPLC-ELSD法测定当归补血汤配方颗粒中黄芪甲苷含量的分析方法简便、准确、重现性好。
OBJECTIVE To establish an HPLC-ELSD method for the determination of Astragaloside IV in Dangguibuxuetang Formula Granules. Methods The Agilent Zorbax C18 column (4.6 mm × 250 mm, 5-Micron) was used with acetonitrile-water as mobile phase at a flow rate of 1.0 mL / min. The column temperature was set at 25 ℃. The ELSD detector drift tube was set at 70 ℃. Set gas flow rate: 2.0 L / min. Results The linear relationship, precision, repeatability and stability of astragaloside Ⅳ under these conditions were good. The average recovery was 97.17%. Conclusion The HPLC-ELSD method for the determination of Astragaloside in Dangguibuxuetang Formula Granules is simple, accurate and reproducible.