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动植物共生菌是药物发现的重要来源之一。为了获取结构新颖且具有良好生物活性的微生物次生代谢产物,本文利用划线分离方法从蜈蚣肠道中分离获得一株共生菌WG4(未鉴定),经土豆液体发酵培养,发酵液经乙酸乙酯萃取、浓缩,获得次级代谢产物浸膏。浸膏经硅胶柱层析、高效液相色谱等分离方法反复纯化,获得3个化合物。通过核磁共振谱和低分辨质谱测试,3个化合物分别鉴定为1-羟基吩嗪(1)、吩嗪-1-甲酰胺(2)和吩嗪-1-羧酸(3)。据文献报道,吩嗪化合物具有抗菌、抗虫、抗病毒、抗肿瘤等生物活性,本实验证实了动植物来源的共生菌具有生产药源化合物的潜力。
Symbiotic flora and fauna is one of the important sources of drug discovery. In order to obtain a novel and well-biologically active secondary metabolites of microorganisms, a symbiotic strain WG4 (unidentified) was isolated from the intestinal tract of centipede by streak separation method. After fermentation by potato liquid, the fermentation broth was extracted with ethyl acetate Extraction, concentration, access to secondary metabolites extract. Extract by silica gel column chromatography, high performance liquid chromatography and other separation methods repeatedly purified to obtain three compounds. Three compounds were identified as 1-hydroxyphenazine (1), phenazine-1-carboxamide (2) and phenazine-1-carboxylic acid (3) respectively by NMR and low resolution mass spectrometry. According to the literature, phenazine compounds have antibacterial, anti-insect, anti-virus, anti-tumor and other biological activity. This experiment confirmed that the plant and animal-derived commensal bacteria have the potential to produce drug-derived compounds.