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纳米材料的合成、结构功能特性及其应用的研究成为人们共同关注的前沿课题.CeO_2是一种廉价而用途极广的材料,如用于发光材料、催化剂、电子陶瓷等.细胞色素c是一种含血红素的金属蛋白质分子,通过对其电化学行为的研究,为认识生物体内的电子传递反应机理和能量转换提供有用信息,对于揭示生命现象的本质具有重大意义.细胞色素c在裸金电极上是极不可逆的,现已发现了加速其可逆反应的多种促进剂,对其电化学反应机理也进行了深入的讨论.本文用溶胶-凝胶法合成了CeO_2纳米晶;将CeO_2纳米晶修饰在金电极上研究了细胞色素c的电子传递反应,发现CeO_2纳米晶是一种良好的促进剂.1 样品的制备与测试称取一定量草酸铈(GR),用蒸馏水调成浆状,滴加浓HNO_3(GR)和H_2O_2(AR),完全溶解后加入柠檬酸(GR),于50～70℃时缓慢蒸发形成溶胶,继续加热有大量气泡产生,并有白色凝胶形成,体积膨胀,有大量棕色烟放出.将凝胶于120℃干燥12h,得到淡黄色干凝胶,将其在不同温度下进行热处理,即得到CeO_2纳米晶.用日本理学D/MAX-IIB型X射线衍射仪进行结构分析;用H-600型透射电子显微镜进行粒子形貌分析和大小测定;用BET法测比表面积.
The synthesis, structural and functional properties of nanomaterials and their applications have become the forefront topics of common concern.CeO 2 is a cheap and versatile material, such as luminescent materials, catalysts, electronic ceramics, etc. Cytochrome c is a Heme-containing metal protein molecules, through the study of their electrochemical behavior, provide useful information for understanding the electron transfer reaction mechanism and energy conversion in vivo, which is of great significance to reveal the essence of the phenomenon of life.Cytochrome c in bare gold Electrode is very irreversible, has been found to accelerate the reversible reaction of a variety of accelerators, electrochemical reaction mechanism is also discussed in depth.In this paper, sol - gel synthesis of CeO2 nanocrystals; CeO2 nano Crystal modification studied the electron transfer reaction of cytochrome c on the gold electrode and found that CeO 2 nanocrystal is a good promoter.1 Preparation and Testing of Sample Weigh a certain amount of cerium oxalate (GR) , Concentrated HNO_3 (GR) and H_2O_2 (AR) were added dropwise. When dissolved completely, citric acid (GR) was added and slowly evaporated to form a sol at 50-70 ° C. Color gel formation, volume expansion, a large number of brown smoke release.Gel at 120 ℃ for 12h, to obtain yellowish xerogel, which at different temperatures for heat treatment, to obtain CeO2 nanocrystals with the Japanese rationale D / MAX-IIB X-ray diffractometer for structural analysis; H-600 transmission electron microscopy particle morphology analysis and size determination; BET specific surface area.