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目的:观察活血通督汤对脊髓损伤后脑源性神经营养因子/酪氨酸蛋白激酶受体B(BDNF/TrkB)信号表达的作用,探讨其对脊髓损伤后肢体运动功能恢复的作用和机制。方法:54只SD大鼠随机分为假手术组、模型组、活血通督汤组,每组18只,造模后每日行BBB肢体运动功能评分,干预7d后取材,采用尼氏染色观察神经元形态,电镜观察脊髓微观结构,免疫荧光定位BDNF阳性细胞,Western blot检测BDNF、TrkB和p-TrkB蛋白表达。结果:干预后第3天起,活血通督汤组BBB评分显著高于模型组(P<0.05);尼氏染色和电镜提示活血通督汤可改善脊髓损伤后神经元和髓鞘的结构和形态;活血通督汤可促进脊髓损伤后脊髓前角细胞分泌BDNF,与模型组比较,差异具有统计学意义(P<0.05);活血通督汤可显著促进BDNF蛋白和p-TrkB蛋白表达(P<0.01),但对TrkB蛋白表达无显著作用。结论:活血通督汤促进脊髓损伤后脊髓前角细胞分泌BDNF,促进TrkB受体磷酸化,从而上调BDNF/TrkB信号表达,促进脊髓损伤后神经元存活、再生和轴突再生、再髓鞘化。
Objective: To observe the effect of Huoxuetongtang on the expression of brain-derived neurotrophic factor / tyrosine kinase B (BDNF / TrkB) signal after spinal cord injury, and to explore the mechanism and mechanism of action of Huoxutongsu Tang on motor function recovery after spinal cord injury. Methods: Fifty-four SD rats were randomly divided into sham-operation group, model group and Huoxuetutu decoction group, with 18 rats in each group. BBB physical motor function score was measured daily after modeling. After 7 days of intervention, The morphology of neurons was observed by electron microscopy. The BDNF positive cells were detected by immunofluorescence. The protein expressions of BDNF, TrkB and p-TrkB were detected by Western blot. Results: The BBB score of Huoxuetongtang group was significantly higher than that of model group on the third day after intervention (P <0.05); Nissl staining and electron microscopy suggested that Huoxuentongtang could improve the structure of neurons and myelin after spinal cord injury Huoxuetongdu Decoction could promote the BDNF secretion of spinal cord anterior horn cells after spinal cord injury, and the difference was statistically significant (P <0.05). Huoxue Tongsu Tang could significantly promote the expression of BDNF protein and p-TrkB protein P <0.01), but no significant effect on TrkB protein expression. Conclusion: Huoxuetong decoction can promote the secretion of BDNF and promote TrkB receptor phosphorylation in spinal cord anterior horn cells after spinal cord injury, so as to up-regulate the expression of BDNF / TrkB signal, promote the survival, regeneration and axon regeneration after spinal cord injury, and remyelination .