论文部分内容阅读
[摘要] 目的 研究内毒素脂多糖（LPS）对人牙周膜成纤维细胞TNF-α、IL-6表达的影响。 方法 培养HPLFs细胞，将细胞分为LPS 刺激0 mg/L（对照组）、1 mg/L（实验Ⅰ组）、10 mg/L（实验Ⅱ组），应用ELISA法检测IL-6、TNF-α因子的表达情况。 结果 LPS 刺激6 h时，HPLFs中TNF-α、IL-6的表达量显示：Ⅰ、Ⅱ组显著高于对照组，差异有统计学意义（P<0.05）。LPS 刺激12 h时，HPLFs中TNF-α、IL-6的表达量显示：Ⅰ、Ⅱ组也显著高于对照组，差异有高度统计学意义（P<0.01）。HPLFs细胞中TNF-α、IL-6的表达随LPS刺激浓度呈剂量依赖性；且对照组、及Ⅰ组、Ⅱ组经LPS 刺激12 h的TNF-α、IL-6表达均显著高于刺激6 h，差异有高度统计学意义（P<0.01）。 结论 内毒素脂多糖（LPS）能够刺激人牙周膜成纤维细胞分泌TNF-α、IL-6炎症因子。
[关键词] 内毒素脂多糖（LPS）；人牙周膜成纤维细胞；TNF-α；IL-6
[中图分类号] R780.2 [文献标识码] B [文章编号] 1673-9701（2016）10-0001-03
[Abstract] Objective To study the effect of lipopolysaccharide（LPS） on the expression of TNF-α and IL-6 in human periodontal fibroblast cells. Methods HPLFs cells were cultured. The cells were divided into LPS stimulated 0 mg/L （control group）， 1 mg/L（experimental group Ⅰ） and 10 mg/L（experimental group Ⅱ）. The expression of IL-6 and TNF-α was detected by ELISA method. Results The expression of LPS， TNF-α and IL-6 in HPLFs was significantly higher than that in control group at 6 hours， the expression of group Ⅱ and group Ⅰ was significantly higher than that in control group， the difference was statistically significant（P<0.05）. The expression of I and TNF-in HPLFs was significantly higher than that in control group， the difference was statistically significant in the expression group Ⅱ and IL-6 in LPS in 12 hours（P<0.01）. The expression of TNF-α and IL-6 in HPLFs cells with LPS stimulus concentration in a dose-dependent manner； and the control group， and group Ⅰ， group Ⅱ by LPS stimulation the expressions of 12 hours of TNF-α and IL-6 were significantly higher than those in the 6 h of stimulation， the difference was statistically significant（P<0.01）. Conclusion Lipopolysaccharide（LPS） can stimulate the secretion of TNF-α and IL-6 inflammatory factors in human periodontal fibroblast cells.
[Key words] Lipopolysaccharide lipopolysaccharide （LPS）； Human periodontal fibroblast； TNF-α； IL-6
革蘭阴性菌的内毒素脂多糖（Lipopolysaccharide，LPS）是一种公认的重要的牙周病致病因子，研究发现，其具有调节细胞的生长、合成代谢，并刺激巨噬细胞、单核细胞、成纤维细胞等分泌IL-6、TNF-α等多种炎症介质的作用[1]。牙周膜成纤维细胞（human periodontal fibroblast，HPLFs）是牙周组织中数量最多的细胞，牙周膜成纤维细胞（HPLFs）在牙周组织健康中发挥着重要作用[2]。本实验旨在探讨经脂多糖刺激后的牙周膜成纤维细胞炎症因子IL-6、TNF-α表达情况，现报道如下。
1 材料与方法
1.1 一般材料
DMEM培养液（Gibco，USA）；牛血清（Gibco，USA）；LPS从细菌Salmonella minnesota Re595中提取并保存。鼠SP免疫组化试剂盒、DAB显色试剂盒；CO2培养箱（Yamato，Japan）；超净工作台（苏州安泰空气技术公司）；倒置显微镜（广西梧洲市光学仪器厂）；酶标分析仪（Thermo corporation，USA）；多功能真彩色细胞图像分析管理系统（Media Cybernetics，USA）。肿瘤坏死因子-α（TNF-α）ELISA 试剂盒（R
[关键词] 内毒素脂多糖（LPS）；人牙周膜成纤维细胞；TNF-α；IL-6
[中图分类号] R780.2 [文献标识码] B [文章编号] 1673-9701（2016）10-0001-03
[Abstract] Objective To study the effect of lipopolysaccharide（LPS） on the expression of TNF-α and IL-6 in human periodontal fibroblast cells. Methods HPLFs cells were cultured. The cells were divided into LPS stimulated 0 mg/L （control group）， 1 mg/L（experimental group Ⅰ） and 10 mg/L（experimental group Ⅱ）. The expression of IL-6 and TNF-α was detected by ELISA method. Results The expression of LPS， TNF-α and IL-6 in HPLFs was significantly higher than that in control group at 6 hours， the expression of group Ⅱ and group Ⅰ was significantly higher than that in control group， the difference was statistically significant（P<0.05）. The expression of I and TNF-in HPLFs was significantly higher than that in control group， the difference was statistically significant in the expression group Ⅱ and IL-6 in LPS in 12 hours（P<0.01）. The expression of TNF-α and IL-6 in HPLFs cells with LPS stimulus concentration in a dose-dependent manner； and the control group， and group Ⅰ， group Ⅱ by LPS stimulation the expressions of 12 hours of TNF-α and IL-6 were significantly higher than those in the 6 h of stimulation， the difference was statistically significant（P<0.01）. Conclusion Lipopolysaccharide（LPS） can stimulate the secretion of TNF-α and IL-6 inflammatory factors in human periodontal fibroblast cells.
[Key words] Lipopolysaccharide lipopolysaccharide （LPS）； Human periodontal fibroblast； TNF-α； IL-6
革蘭阴性菌的内毒素脂多糖（Lipopolysaccharide，LPS）是一种公认的重要的牙周病致病因子，研究发现，其具有调节细胞的生长、合成代谢，并刺激巨噬细胞、单核细胞、成纤维细胞等分泌IL-6、TNF-α等多种炎症介质的作用[1]。牙周膜成纤维细胞（human periodontal fibroblast，HPLFs）是牙周组织中数量最多的细胞，牙周膜成纤维细胞（HPLFs）在牙周组织健康中发挥着重要作用[2]。本实验旨在探讨经脂多糖刺激后的牙周膜成纤维细胞炎症因子IL-6、TNF-α表达情况，现报道如下。
1 材料与方法
1.1 一般材料
DMEM培养液（Gibco，USA）；牛血清（Gibco，USA）；LPS从细菌Salmonella minnesota Re595中提取并保存。鼠SP免疫组化试剂盒、DAB显色试剂盒；CO2培养箱（Yamato，Japan）；超净工作台（苏州安泰空气技术公司）；倒置显微镜（广西梧洲市光学仪器厂）；酶标分析仪（Thermo corporation，USA）；多功能真彩色细胞图像分析管理系统（Media Cybernetics，USA）。肿瘤坏死因子-α（TNF-α）ELISA 试剂盒（R