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【目的】检测玫瑰红鹅膏中所含肽类毒素及其含量,并对其肽类毒素的抑制白色念珠菌活性进行研究。【方法】采用HPLC和ESI-MS法从玫瑰红鹅膏中分离并鉴定出所含肽类毒素,并采用HPLC法测定其子实体、菌盖及菌柄和菌托混合部分中肽类毒素的含量。同时,采用纸片法研究了玫瑰红鹅膏粗毒液和分离到的单品肽类毒素对白色念珠菌JLC31680和JLC31681的抑菌作用。【结果】分离并鉴定出α-鹅膏毒肽(α-AMA)、β-鹅膏毒肽(β-AMA)和二羟鬼笔毒肽(PHD)等3种肽类毒素。玫瑰红鹅膏子实体中α-AMA、β-AMA、PHD的含量分别为30.3168、6.9932和9.9459 mg/g;菌盖中含量分别为44.9573、11.0798和11.3025 mg/g;菌柄和菌托混合部分中:α-AMA 11.6904 mg/g和PHD 7.9775 mg/g,β-AMA未检出。粗毒液、α-AMA、β-AMA和PHD对白色念珠菌JLC31680均具有很好的抑制作用,抑制率分别达到11.96%、32.52%、23.29%(p<0.01)和15.46%(p<0.05);粗毒液和β-AMA对白色念珠菌JLC31681的最高抑制率分别为10.16%和11.10%(p<0.01),α-AMA对白色念珠菌JLC31681最高抑菌率为6.89%(p<0.05)。【结论】玫瑰红鹅膏中的三种肽类毒素的含量较高,是制备肽类毒素的新资源;其具有抑制白色念珠菌的活性,可开发利用。
【Objective】 The aim of the study was to determine the content and content of peptide toxins contained in Rose Amanita and the inhibitory activity of peptide toxins against Candida albicans was studied. 【Method】 Peptide toxins were isolated and identified by HPLC and ESI-MS from rose red amanitaine. The contents of peptide toxins were determined by HPLC from their fruiting body, cap, and mixture of stipe and stalk content. At the same time, the antibacterial activity of Rose Red Amanita venom and the isolated single peptide toxins on Candida albicans JLC31680 and JLC31681 were studied by the paper method. 【Result】 Three peptide toxins, α-AMA, β-AMA and PHD, were isolated and identified. The contents of α-AMA, β-AMA and PHD in the rose red goose angels were 30.3168, 6.9932 and 9.9459 mg / g, respectively. The content in the cap was 44.9573, 11.0798 and 11.3025 mg / g, respectively. In the section: α-AMA 11.6904 mg / g and PHD 7.9775 mg / g, β-AMA undetectable. The inhibitory rates of crude venom, α-AMA, β-AMA and PHD were 11.96%, 32.52%, 23.29% (p <0.01) and 15.46% (p <0.05) for Candida albicans JLC31680, The maximum inhibitory rates of crude venom and β-AMA against Candida albicans JLC31681 were 10.16% and 11.10%, respectively (p <0.01). The highest inhibitory rate of α-AMA against Candida albicans JLC31681 was 6.89% (p <0.05). 【Conclusion】 The content of three peptide toxins in Rose Red Amanita is high, which is a new resource for preparation of peptide toxins. It has the activity of inhibiting Candida albicans and can be exploited and utilized.