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目的 探索脑血管平滑肌细胞c-fos早期快反应基因与脑血管痉挛的关系。方法 新西兰兔36只,随机等分为3组:实验组、新霉素组和对照组。实验组和新霉素组采用二次注血模型,新霉素的静脉注射给药剂量根据预实验确定为3mg·kg-1,每天2次。分别于12h、2d和7d处死动物,脑标本经灌注固定后行c-Fos免疫组化染色。结果 实验组c-fos早期快反应基因的表达强度（75％）明显高于新霉素组（25％）和对照组（17％）（P=0.032）,c-Fos表达在蛛网膜下腔出血后2d内最显著,并持续7d以上,而血管性病理损害发生在2d以后（P=0.005）。结论 c-fos早期快反应基因与蛛网膜下腔出血后脑血管痉挛病理改变有关。
Objective To explore the relationship between c-fos early rapid response gene and cerebral vasospasm in cerebrovascular smooth muscle cells. Methods 36 New Zealand rabbits were randomly divided into three groups: experimental group, neomycin group and control group. The experimental group and the neomycin group with a second injection model, intravenous injection of neomycin dose according to preliminary experiments to determine 3mg · kg-1, 2 times a day. Animals were killed at 12h, 2d and 7d, respectively. The brain samples were fixed by perfusion and c-Fos immunohistochemical staining. Results The expression of c-fos early rapid response gene (75%) in experimental group was significantly higher than that in neomycin group (25%) and control group (17%) (P = 0.032) 2d after bleeding the most significant, and continued for more than 7d, and vascular pathological damage occurred after 2d (P = 0.005). Conclusions Early c-fos fast response gene is associated with the pathological changes of cerebral vasospasm after subarachnoid hemorrhage.