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目的:考察雪上一枝蒿药材及其制剂发挥镇痛作用的治疗窗范围,并比较它们之间的毒性大小。方法:采用经典的急性毒性试验方法进行雪上一枝蒿及其制剂的急性致死量试验,醋酸扭体法进行镇痛药效试验,采用SPSS17.0统计软件计算急性毒性的LD50、LD5和发挥镇痛药效的ED50、ED95,求得各组别发挥镇痛作用的治疗指数(TI),安全系数(SF),以此评价雪上一枝蒿药材及其制剂发挥镇痛作用的有效性和安全性。结果:各组别发挥镇痛作用的治疗窗有所不同,药材醇提液、总生物碱溶液、总生物碱微乳发挥镇痛作用的治疗指数TI分别为5.250、6.825、10.460,安全系数SF分别为1.178、1.650、1.788。结论:雪上一枝蒿总生物碱微乳与总生物碱溶液、雪上一枝蒿药材醇提液相比,其治疗窗较宽。三者的治疗窗大小为:总生物碱微乳>总生物碱溶液>药材醇提液。
OBJECTIVE: To investigate the therapeutic window of analgesic effect of Artemisia annua and its preparations, and to compare the toxicity between them. Methods: The classical acute toxicity test was used to test the acute lethal dose of Artemisia annua and its preparations. The acetic acid writhing method was used to test the analgesic efficacy. SPSS17.0 statistical software was used to calculate acute toxicity of LD50 and LD5 and exert analgesia Efficacy and ED50, ED95, find the treatment index (TI) and safety factor (SF) of the analgesic effect of each group, to evaluate the efficacy and safety of analgesic effect of Artemisia selengensis. Results: The therapeutic window of analgesic effect of each group was different. The therapeutic indexes TI of analgesic effect of alcohol extract, total alkaloid solution and total alkaloid microemulsion were 5.250, 6.825 and 10.460 respectively. The safety factor SF Respectively 1.178,1.650,1.788. Conclusion: Compared with total alkaloid solution and Artemisia annua L. alcohol extract, the therapeutic window of Artemisia annua alkaloids is wider. The three treatment window size: total alkaloid microemulsion> total alkaloid solution> medicinal alcohol extract.