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目的 研究肝切除对种植性肝癌的生长、增殖细胞核 (PCNA)抗原和细胞癌基因 (c fos)的表达的作用及以术后应用维甲酸在这一过程的作用。方法 大鼠分为 5组 :①单纯行左肝切除术 ;②单纯右肝种植肿瘤 ;③左肝切除加单纯右肝种植肿瘤 ;④左肝切除加右肝种植肿瘤术后应用维甲酸 ;⑤单纯左肝切除术 ,术后应用维甲酸。 6天取①⑤全组、②③④组各一半处死 ,右心房采血测量ALA及GPT水平 ,剖腹测量肿瘤大小。取肿瘤组织及距肿瘤组织 1 5～ 2cm远的肝中叶组织 ,观察增殖细胞抗原及癌基因c fos表达情况。②③④组其余大鼠观察其生存时间的差异。结果 ①肝切除能促进种植性肝癌的生长及增生 ;②肝切除可促进癌基因c fos的表达 ;③肿瘤的存在可抑制肝细胞的再生 ;④术后应用维甲酸可抑制这一现象并可延长肝切除后种植性肝癌大鼠的生存时间。结论 维甲酸对肝切除后肝脏的再生、肝功能的恢复无明显的影响
Objective To investigate the effect of hepatectomy on the growth of implanted hepatocellular carcinoma (HCC), the expression of proliferating cell nuclear antigen (PCNA) and oncogene (c fos), and the role of retinoic acid in this process. Methods The rats were divided into 5 groups: ① simple left hepatectomy; ② pure right hepatic tumor; ③ left hepatectomy plus pure right hepatic tumor; ④ left hepatectomy plus right hepatic tumor after application of retinoic acid; Simple left hepatectomy, retinoic acid after surgery. 6 days to take ① ⑤ the whole group, ② ③ ④ group were sacrificed by half, ALA and GPT levels were measured by right atrium blood sampling, tumor size was measured by Caesarean section. The tumor tissues and the middle lobe of the liver 1 to 5 cm away from the tumor tissues were taken to observe the expression of proliferating cell antigen and oncogene c fos. ② ③ ④ the remaining rats observed differences in their survival time. Results ① Hepatectomy can promote the growth and proliferation of implanted hepatocellular carcinoma; ② Hepatectomy can promote the expression of oncogene c fos; ③ The existence of tumor can inhibit the regeneration of hepatocytes; ④ After application, retinoic acid can inhibit this phenomenon and Prolong the survival time of hepatocellular carcinoma in rats after liver resection. Conclusion Retinoic acid has no obvious effect on liver regeneration and liver function recovery after liver resection