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测定益母草多糖含量和研究其多糖的抗氧化性。利用水提醇沉法提取粗多糖,并利用Sevage法脱蛋白对其进行纯化;采用苯酚-硫酸法测定益母草多糖样品中纯多糖的含量并采用邻苯三酚自氧化法和水杨酸法分别研究益母草多糖清除O2-·和·OH的效果。益母草提取所得多糖样品中纯多糖含量达48·5%,益母草多糖具有较强的清除O2-·和·OH的作用。
Determination of the content of Leonurus polysaccharide and study its antioxidant activity of polysaccharides. Crude polysaccharides were extracted by water extraction and alcohol precipitation and purified by Sevage method. The content of pure polysaccharides was determined by phenol-sulfuric acid method and pyrogallol autoxidation method and salicylic acid method Study the effect of Motherwort polysaccharide on O2- · and · OH removal. The content of pure polysaccharides in the polysaccharides extracted from Motherwort reached 48.5%, and the motherwort polysaccharides had a strong ability to scavenge O2- and · OH.