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目的:观察土荆芥挥发油对幽门螺杆菌(Hp)感染小鼠的体内Hp清除作用及对胃黏膜组织NF-κB表达水平的影响。方法:44只SPF级昆明小鼠采用经口感染方法建立Hp感染模型,随机分为空白组、模型组、三联组、土荆芥组。空白组不予干预,模型组给予灭菌注射用水,三联组予兰索拉唑+甲硝唑+克拉霉素给药1周,土荆芥组给予土荆芥挥发油4周,所有动物均于给药4周后处死。快速尿素酶试验、组织病理学染色判定Hp根除情况,免疫组织化学方法测定胃黏膜组织NF-κB p65水平。结果:三联组Hp根除率为70%(7/10),土荆芥组为50%(5/10),两组比较差异无统计学意义(Fisher精确概率法P=0.65)。模型组细胞核NF-κB p65平均光密度及细胞核/细胞浆比值较空白组显著升高(P<0.05,P<0.01),治疗后三联组及土荆芥组NF-κB p65有降低趋势,但差异均无统计学意义。结论:土荆芥具有根除Hp的作用,其对胃黏膜组织NF-κB入核过程可能有一定程度的抑制作用。
OBJECTIVE: To observe the Hp scavenging effect of the essential oil of Schisandra chinensis in mice infected with Helicobacter pylori (Hp) and the effect on the expression of NF-κB in gastric mucosa. Methods: Forty-four SPF Kunming mice were randomly divided into blank group, model group, triple group and soil Nepeta group by oral infection. In the blank group, no intervention was given. The model group was given sterile water for injection. The triple group was given lansoprazole + metronidazole + clarithromycin for 1 week. The group Nepeta was given the essential oil for 4 weeks. All the animals were treated with Four weeks after sacrifice. Rapid urease test, histopathological staining to determine the eradication of Hp, gastric mucosal tissue by immunohistochemistry NF-κB p65 levels. Results: The Hp eradication rate was 70% (7/10) in the triple group and 50% (5/10) in the Nepeta group. There was no significant difference between the two groups (Fisher exact test, P = 0.65). The mean optical density of NF-κB p65 and the ratio of nucleus to cytoplasm in nucleus pulposus in model group were significantly higher than those in blank group (P <0.05, P <0.01) The difference was not statistically significant. Conclusion: Nepeta has the effect of eradicating Hp, which may inhibit the NF-κB entry into gastric mucosa to a certain degree.