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目的了解福州海港口岸鼠形动物巴尔通体感染状况,并掌握其菌种基因类型、遗传特征和变异关系,为该菌种的溯源及鼠传疾病防治工作提供科学依据。方法 2013年2月—2014年1月,随机抽取福州海港口岸4个监测点,采用鼠笼法捕获鼠形动物,解剖取其肝、肾,研磨后提取核酸,进行PCR扩增,并对所得产物进行测序及系统进化分析。结果共捕获212只鼠形动物,经分类鉴定,隶属于2目2科3属4种,以黄胸鼠为优势种群,占39.62%,臭鼩鼱和褐家鼠分别占33.96%和23.59%。鼠密度2.31%。2只雄性黄胸鼠检出巴尔通体,感染率为0.94%。遗传进化分析显示本次检测到的巴尔通体与B.taylorii以及对人类有致病性的B.tribocorum的遗传关系最近。结论福州海港口岸鼠形动物中存在巴尔通体感染,而且携带人类致病性巴尔通体,存在人群感染的风险。
Objective To understand the infectious status of Bartonella Bartonella in Fuzhou harbor port and to understand the genotypes, genetic characteristics and variation of the species of Staphylococcus aureus at the port of Fuzhou, so as to provide a scientific basis for the traceability and prevention and cure of the disease. Methods From February 2013 to January 2014, four monitoring points at the port of Fuzhou were randomly selected. Murine animals were captured by squirrel-cage method. Liver and kidney were dissected and their nucleic acids were extracted after grinding. The PCR products were obtained by PCR. Product sequencing and phylogenetic analysis. Results A total of 212 mouse-shaped animals were identified and classified. They belonged to 4 genera, 2 genera, 2 families, 3 genera and 2 dominant species. The dominant species was Rattus flavipectus, accounting for 39.62%, 33.96% and 23.59% . Rat density 2.31%. Two male Rattus flavipectus detected Bartonella, the infection rate was 0.94%. Genetic evolution analysis showed that the genetic relationship between Bartonella body detected in this study and B. tylorii and B.tribocorum which is pathogenic to humans was the most recent. Conclusions There is Bartonella in rats in Fuzhou harbor port, and carries human pathogenic Bartonella, which may lead to the risk of human infection.