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利用自聚合法合成聚乙烯吡咯烷酮包金(Au@PVP)核壳纳米粒子,通过氢键作用使酸性橙Ⅱ吸附在Au@PVP核壳纳米粒子SERS基底上,优化激发光波长,选择合适的测试条件,利用金纳米内核的等离子体共振效应实现对酸性橙Ⅱ分子的高灵敏度检测,在10~(-4)~10~(-10)mol/L浓度范围内呈现线性关系,相关系数0.94,检测限可达到10~(-11)mol/L。对橙汁饮料进行加标处理,采用甲醇提取并经过微孔滤膜过滤得到提取液,样品回收率在77%~104.6%,相对标准偏差在3.88%~8.46%。相较于《中国出入境检验检疫标准》SN/T 3536-2013,本方法检测限降低近4个数量级。
The Au @ PVP core-shell nanoparticles were synthesized by the self-polymerization method. Acid Orange Ⅱ was adsorbed on the SERS substrate of Au @ PVP core-shell nanoparticles through hydrogen bonding, and the wavelength of the excitation light was optimized. The appropriate test Under the condition of 10 ~ (-4) ~ 10 ~ (-10) mol / L, the linearity of the molecule was detected by the plasmon resonance of gold nanocrystals. The correlation coefficient was 0.94, The detection limit can reach 10 ~ (-11) mol / L. The orange juice beverage was spiked and extracted with methanol and filtered through a microporous membrane filter to obtain the extract. The recoveries of the samples ranged from 77% to 104.6% with relative standard deviations of 3.88% to 8.46%. Compared with “China Entry-Exit Inspection and Quarantine Standards” SN / T 3536-2013, the detection limit of this method is reduced by nearly 4 orders of magnitude.