干旱是一种重要的非生物胁迫形式,农作物产量受干旱影响巨大。甘蔗是一种重要的经济和能源作物,而目前对于甘蔗干旱响应调节过程知之甚少。本研究利用高通量测序技术,对甘蔗品种新台糖22在伸长期的抗旱机理进行研究,发现6 861个上调基因和4 574个下调基因,并发现存在大量差异表达基因参与代谢和响应刺激等生物学过程。另外本研究还发现了一系列参与水分和氧化胁迫响应的基因,并找到许多起调节作用的转录因子和蛋白激酶,最后利用定量PCR证明测序结果的可靠性。 Drought is an important form of abiotic stress and crop yields are greatly affected by the drought. Sugarcane is an important economic and energy crop, and little is known about the regulation of sugarcane drought response. In this study, a high-throughput sequencing technique was used to study the drought resistance mechanism of sugarcane variety Tiantang 22 in the elongation stage. There were 6,861 up-regulated genes and 4 574 down-regulated genes, and found a large number of differentially expressed genes involved in metabolism and response stimulation Biological process. In addition, this study also found a series of genes involved in the response to water stress and oxidative stress and found many transcription factors and protein kinases that play a regulatory role. Finally, the reliability of the sequencing results was confirmed by quantitative PCR.