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从对照和用ＤＥＨＰ处理的大鼠肝脏提取核蛋白，以含酰基ＣｏＡ氧化酶（ＡＯＸ）基因表达调控部位的ＤＮＡ片段和该基因的不同蛋白结合位点的ＤＮＡ片段作为核蛋白结合反应的探针，通过凝胶电泳迁移率改变实验和Ｓｏｕｔｈｗｅｓｔｅｒｎ印迹分析检查了ＤＥＨＰ对ＡＯＸ基因反式作用因子的影响。结果表明，降血脂药物ＤＥＨＰ可显著增加ＡＯＸ基因反式作用因子的含量和（或）与基因的结合活性，在转录水平上促进基因的表达。
Nucleoproteins were extracted from control and DEHP-treated rat livers. DNA fragments containing the acyl CoA oxidase (AOX) gene expression control site and DNA fragments of different protein binding sites of the gene were used as probes for the nuclear protein binding reaction , The effects of DEHP on the trans-acting factors of the AOX gene were examined by gel electrophoretic mobility shift experiments and Southwestern blot analysis. The results showed that the lipid-lowering drug DEHP can significantly increase the AOX gene trans-acting factor content and / or the binding activity with the gene, and promote the gene expression at the transcriptional level.