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目的观察白介素-18(IL-18)及信号转导和转录激活因子5(STAT5)在4～24周糖尿病大鼠视网膜的表达,初步探讨其在糖尿病视网膜病变(DR)中可能的分子机制。方法用限制性片段差异显示聚合酶链反应(RFDD-PCR)技术,建立正常大鼠和糖尿病8周大鼠视网膜基因表达谱。以生物信息学分析两者差异,筛选出候补基因IL-18及STAT5。以半定量反转录聚合酶链反应(RT-PCR)观察IL-18及STAT5在4、8、24周糖尿病大鼠视网膜的表达。结果RFDD-PCR结果显示,正常组IL-18表达明显强于糖尿病组;正常组STAT5无表达,糖尿病组较强表达。RT-PCR结果显示,与正常相比,糖尿病4周时,视网膜高表达IL-18,8周IL-18表达降低,24周表达最低。STAT5在正常及糖尿病4周视网膜未见表达;8周开始表达,24周时最强。结论IL-18表达变化及STAT5的活化与DR发生有关。IL-18的表达不依赖于STAT5的活化。
Objective To investigate the expression of interleukin-18 (IL-18) and signal transducers and activators of transcription 5 (STAT5) in the retina of diabetic rats for 4 ~ 24 weeks and to explore its possible molecular mechanism in diabetic retinopathy. Methods Retinal gene expression profiles in normal rats and diabetic rats for 8 weeks were established by restriction fragment differential display polymerase chain reaction (RFDD-PCR). Bioinformatics analysis of the difference between the two, screening out candidate genes IL-18 and STAT5. Retinal expression of IL-18 and STAT5 in 4,8,24-week diabetic rats was observed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results The results of RFDD-PCR showed that the expression of IL-18 in normal group was significantly stronger than that in diabetic group; STAT5 was not expressed in normal group, but was significantly higher in diabetic group. RT-PCR results showed that IL-18 expression in the retina was significantly increased at 4 weeks after diabetes mellitus, and IL-18 expression was lower at 8 weeks and lowest at 24 weeks. STAT5 in the normal and diabetic retinal 4 weeks no expression; 8 weeks began to express the strongest at 24 weeks. Conclusion The change of IL-18 expression and the activation of STAT5 are related to the occurrence of DR. The expression of IL-18 is independent of STAT5 activation.