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目的改进仪器自动化批量制备全血流程及方法,建立适合本中心成分血自动化制备方法。方法回顾性分析手工法(方法 1)、改进前仪器法(方法 2)及改进后仪器法(方法 3)分离全血制备红细胞和血浆的各参数,包括批次不同数量全血及人员组合时3种方法的效率、产品质量等,分析血液质量影响,比较工作效率。结果 3种方法血液外观质量及全项质量抽检均符合国家标准要求。所有组合中方法 3效率均高于方法 2,差异具统计学意义。除了2人组制备25-60袋全血外,其余组合中方法 3效率高于方法 1,差异具统计学意义。在3人组、4人组制备25-60袋、61-120袋全血的组合中,方法 1效率高于方法 2,差异均具统计学意义,但在3人组和4人组制备>120袋、2人组制备25-60袋全血的组合中,方法 1与方法 2效率差异不具统计学意义。结论使用方法 3进行批量全血制备能够有效提高工作效率且血液质量符合国家标准要求,适合本中心。
OBJECTIVE To improve the automatic preparation of whole blood processes and methods for instrument automation and to establish an automated method for the preparation of blood suitable for this center. Methods The parameters of erythrocytes and plasma isolated from whole blood by manual method (method 1), modified instrument method (method 2) and modified instrument method (method 3) were retrospectively analyzed, including different batches of whole blood and human combination 3 methods of efficiency, product quality, analysis of the impact of blood quality, work efficiency. Results The three methods of blood appearance quality and quality of all sampling are in line with national standards. The efficiency of method 3 in all combinations was higher than that of method 2, with statistically significant differences. In addition to the 2-person group of 25-60 bags of whole blood preparation, the other combinations of method 3 is more efficient than the method 1, the difference was statistically significant. In the 3-person and 4-person groups, 25-60 bags and 61-120 bags of whole blood were prepared, and the efficiency of the method 1 was higher than that of the method 2, the difference was statistically significant, but in the 3-person group and the 4-person group, 120 bags, two people in the preparation of 25-60 bags of whole blood combination, the efficiency of methods 1 and 2 was not statistically significant. Conclusion The use of method 3 for batch whole blood preparation can effectively improve the work efficiency and blood quality in line with national standards, suitable for the center.