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目的研究医院临床分离的耐甲氧西林金黄色葡萄球菌(MRSA)的耐药表型和消毒剂抗性基因携带情况,为加强MRSA感染防控提供理论依据。方法采用细菌分离培养方法和PCR技术,对徐州地区医院住院患者送检病原学标本分离的MRSA耐药表型和抗消毒剂基因携带情况进行检测。结果从临床标本分离的280株金黄色葡萄球菌中共鉴定出MRSA 157株,占检出金黄色葡萄球菌的56.1%。临床分离MRSA仅对万古霉素、奎奴普丁/达福普汀和利奈唑胺敏感,对其他所试验的抗菌药物普遍耐药。MRSA菌株中有28.0%携带抗消毒剂基因qac A/B,MSSA菌株中携带qac A/B基因仅占7.3%。结论该地区医院患者感染的金黄色葡萄球菌中MRSA检出率较高,其MRSA菌株携带抗消毒剂基因比例明显高于敏感株,且普遍耐药,应加强MRSA监测和控制。
Objective To study the drug resistance phenotypes and disinfectant resistance genes of methicillin-resistant Staphylococcus aureus (MRSA) clinically isolated in the hospital and provide a theoretical basis for prevention and control of MRSA infection. Methods Bacterial isolation and culture methods and PCR techniques were used to detect MRSA resistant phenotype and anti-disinfectant gene carriers in hospitalized patients in Xuzhou. Results A total of 157 strains of MRSA were identified among the 280 Staphylococcus aureus isolated from clinical specimens, accounting for 56.1% of the total S. aureus detected. MRSA was clinically sensitive only to vancomycin, quinupristin / dalfopristin, and linezolid, and was generally resistant to other antibiotics tested. 28.0% of MRSA strains carried qac A / B, an anti-disinfectant gene, and 7.3% of MSSA strains carried qac A / B gene. Conclusion The prevalence of MRSA in hospital-acquired Staphylococcus aureus is high. MRSA strains carry a significantly higher proportion of anti-disinfectant genes than susceptible strains, and are generally resistant to MRSA. MRSA surveillance and control should be strengthened.