原发性闭经是妇科难解决的问题。作者经过18个月系统的分析、试验,用“Y”染色体荧光试验进行诊断。确诊原发性闭经可分为六步:即全身检查、颊粘膜刮片、口服孕激素水平、尿中总促性腺激素、“Y”染色体荧光测定,及细胞核的形态。用颊粘膜刮片鉴别,可以分为染色质阳性及阴性两组。颊粘膜刮片染色质阳性者,包括有卵巢肿瘤,原发性卵巢功能衰竭,性机能减退的类似无睾症,及神经性肿瘤等。这些症状可以用黄体酮撤退出血作进一步鉴别。若撤退 Primary amenorrhea is difficult to solve gynecological problems. After 18 months of systematic analysis and testing, the authors used the “Y” chromosome fluorescence test for diagnosis. Confirmed primary amenorrhea can be divided into six steps: the body examination, buccal mucosal scraping tablets, oral progesterone levels, urinary total gonadotropin, “Y” chromosome fluorescence determination, and nuclear morphology. With buccal mucosal smear identification, can be divided into two groups of positive and negative chromatin. Buccal mucosal smear positive for those with chromatin, including ovarian tumors, primary ovarian failure, similar to the absence of sexual dysfunction, and neurogenic tumors. These symptoms can be further identified with progesterone withdrawal bleeding. If retreat