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目的建立反相高效液相色谱(RP-HPLC)法测定枯草杆菌二联活菌颗粒中甜菊糖苷的含量。方法选用Agi Lent Zobax SB-C18(250 mm×4.6 mm,5μm)为色谱柱,线性梯度洗脱,流动相为乙腈-0.002 mol/L磷酸溶液;检测波长为210 nm;流速1.0 ml/min;进样量10μl;柱温30℃。结果甜菊糖苷的线性范围为5.160~103.2 g/ml(r=0.9999,n=7);提取回收率为99.04%(RSD为0.6%,n=6)。结论本法操作简便、准确,可用于枯草杆菌二联活菌颗粒的质量控制。
Objective To establish an RP-HPLC method for the determination of stevioside in B. subtilis. Methods Agi Lent Zobax SB-C18 (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase was acetonitrile-0.002 mol / L phosphoric acid solution. The detection wavelength was 210 nm and the flow rate was 1.0 ml / min. Injection volume 10μl; column temperature 30 ℃. Results The linear range of stevioside was 5.160 ~ 103.2 g / ml (r = 0.9999, n = 7). The extraction recovery was 99.04% (RSD = 0.6%, n = 6). Conclusion The method is simple, accurate and can be used for the quality control of B. subtilis viable particles.