目的:分析肝细胞癌相关抗原编码基因HCA520与钙离子的关系.方法:通过PCR获得目的基因,构建至原核表达载体pGEX-4T-3中.在大肠杆菌......

Objective: Tumor associated antigen encoding gene HCA520 (AF146019) was identified by screening a human hepatocellular c......

HCA520是用SEREX(serological identification of recombinant cDNA expressing cloning)方法,即用肝癌病人血清从肝癌组织cDNA表......

目的:研究肿瘤相关抗原HCA520对HEK293细胞增殖的影响,获得HCA520生物学功能的初步提示.方法:构建pcDNA3-HCA520-flag真核表达载体......

应用PCR技术获得肿瘤相关抗原HCA520编码基因，构建至pGEM-T Easy载体，测序正确后，亚克隆至pEGFP-N1载体，1％的琼脂糖电泳得到两条带，与预......