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选 2个不同淀粉类型的早籼品种 ,对水稻开花后籽粒中直、支链淀粉的积累动态及淀粉合成代谢的 3个关键酶— ADPG焦磷酸化酶、可溶性淀粉合成酶与淀粉分支酶活性变化进行分析 ,并采用人工控温试验等方法探讨了不同温度下 ADPG焦磷酸化酶、可溶性淀粉合成酶与淀粉分支酶的活性变化特征。研究结果表明 ,在水稻籽粒灌浆过程中支链淀粉与直链淀粉几乎是同步积累的 ,不同品种的淀粉积累形成差异表现在两者的相对积累比率上 ;上述 3个酶的活性变化在水稻灌浆过程中存在着十分明显时期特点 ,其中可溶性淀粉合成酶的活性在水稻籽粒灌浆初期的活性最高 ,而 ADPG焦磷酸化酶与淀粉分支酶的活性则分别在开花后 15天与 2 0天左右时达到其活性高峰值 ;在籽粒淀粉合成代谢的 3个关键酶中 ,淀粉分支酶 (Q-酶 )活性可能是温度对稻米淀粉品质变化产生影响作用的酶调节位点之一 ,其不同温度下的活性表现也可能存在着一定的品种类型特征。
Two early indica cultivars with different starch types were selected to study the dynamic accumulation of amylopectin and amylopectin and the three key enzymes of starch synthesis and metabolism - ADPG pyrophosphorylase, soluble starch synthase and starch branching enzyme activity The changes of ADPG pyrophosphorylase, soluble starch synthase and starch branching enzyme activities under different temperatures were investigated by artificial temperature control test. The results showed that amylose and amylose accumulated almost simultaneously in the process of rice grain filling. The differences of starch accumulation in different cultivars were found in the relative accumulation ratio of the two cultivars. The changes of the activity of the three enzymes in rice grain filling During the process, there were obvious characteristics of the period. Among them, the activity of soluble starch synthase was the highest in the early grain filling stage, while the activities of ADPG pyrophosphorylase and starch branching enzyme were 15 days and 20 days after anthesis The activity of starch branching enzyme (Q-enzyme) may be one of the enzyme regulating sites that affect the starch quality change of rice at different temperatures The performance of the activity may also exist a certain variety type characteristics.